NAD+ (500mg)
NAD+ (Nicotinamide Adenine Dinucleotide) is a vital coenzyme involved in hundreds of cellular processes. Reconstitute with 5mL of bacteriostatic water for a concentration of 100mg/mL.
Reviewed by the AllPeptides.eu Editorial Team·Last reviewed: 2026-06-16
Educational information. Not medical advice, and no efficacy claim is implied. Regulatory approval is not a use recommendation by AllPeptides.eu.
⚠️ For informational/educational purposes only. Not medical advice. We do not sell any products. Self-treatment carries serious risks — consult a healthcare professional. Disclaimer
Research monograph
See the full NAD+ encyclopedia page with mechanism, evidence tier, related variants and research context.
How It Works
NAD+ is essential for mitochondrial ATP production, DNA repair via PARP, and sirtuin activation. Supplementation reverses aspects of cellular aging.
Quick Start
- Typical dose: 50–100 mg subcutaneously, 3× weekly.
- Frequency: 3×/week (e.g. Mon/Wed/Fri)
- Storage: 2–8°C after reconstitution
Dosing Table — NAD+ 500mg
Route: Subcutaneous injection | Frequency: 3× weekly
| Week | Dose | Units (per injection) |
|---|---|---|
| Weeks 1–2 | 50 mg | 50 Units (0.50 mL) |
| Weeks 3–8 | 100 mg | 100 Units (1.00 mL) |
Subcutaneous administration 3 times weekly (e.g. Mon/Wed/Fri): 50 mg in weeks 1–2, then 100 mg. The injection may cause transient stinging — inject slowly. There is no clinical protocol for subcutaneous NAD+; this schedule reflects reported practice.
Related compounds
Reconstitution Steps
- Clean vial stoppers with an alcohol swab.
- Draw up 5mL of bacteriostatic water.
- Slowly inject into the vial wall.
- Gently swirl until fully dissolved.
- Store refrigerated (2–8°C).
Protocol Overview
- NAD+ declines with age — replenishment supports cellular energy and DNA repair.
- Activates sirtuins (SIRT1-7), key regulators of aging.
- Supports mitochondrial function and metabolism.
Dosing Protocol
- Start: 50 mg 3×/wk (Wks 1–2).
- Maintenance: 100 mg 3×/wk (Wks 3–8).
- Weekly amount: 150 mg (Wks 1–2) → 300 mg.
- Cycle: 4–8 weeks, subcutaneous only.
- Break between cycles: After 4–8 weeks, a 4-week pause before the next cycle.
Washout & Cycle Restart
When can I restart?
After 4–8 weeks, a 4-week pause before the next cycle.
Minimum wait period: 4 weeks
Storage Instructions
- Reconstituted: 2–8°C, use within 21 days.
- Powder: 2-8°C or -20°C long-term.
Important Notes
- Stinging during injection is normal—decreases with slow administration.
- Avoid late-night injections (may disrupt sleep).
- Combines well with TMG (trimethylglycine) for methyl group support.
Potential Benefits & Side Effects
Potential Benefits:
- Increased cellular energy
- Enhanced DNA repair
- Anti-aging via sirtuins
- Improved neurological function
- Mitochondrial health support
Potential Side Effects:
- Injection site stinging/soreness
- Nausea (at high doses)
- Sleep disturbance (if injected late)
- Flushing
⚠️ Serious Warnings & Long-Term Risks
The following warnings are based on published literature, FDA labels, and post-marketing surveillance. They do not constitute medical advice. Consult a healthcare professional.
Metabolism enhancement — potential tumor protection
NAD+ elevation may theoretically benefit cancer cells by enhancing their energy metabolism (Warburg effect). Some studies show that NAMPT inhibition (NAD synthesis enzyme) suppresses oncogenesis.
📄 Cancer Cell 2018; Nat Rev Cancer 2020Flushing & GI discomfort with IV administration
IV NAD+ commonly causes chest tightness, flushing, nausea, cramps. Subcutaneous administration reduces these but with local pain.
📄 GeroScience 2023References
- NAD+ and Aging — Yoshino J, et al. NAD+ intermediates: the biology and therapeutic potential. Cell Metab. 2018;27(3):513-528.
View Source - Sirtuins and NAD+ — Imai S, Guarente L. NAD+ and sirtuins in aging. Trends Cell Biol. 2014;24(8):464-471.
View Source
Recommended Supplements
NMN (Nicotinamide Mononucleotide)
CoQ10 (Ubiquinol)
Vitamin E (Mixed Tocopherols)
External Sources & Regulatory Status
Regulatory Approvals
Dietary supplement (GRAS)
Dietary supplement (GRAS)
Educational information. Not medical advice, and no efficacy claim is implied. Regulatory approval is not a use recommendation by AllPeptides.eu.
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