NAD+ (250mg)
NAD+ (Nicotinamide Adenine Dinucleotide) is a critical coenzyme for cellular metabolism and DNA repair. Research sources report subcutaneous administration of 50 mg to 100 mg, once daily, increased gradually.
Reviewed by the AllPeptides.eu Editorial Team·Last reviewed: 2026-06-16
Educational information. Not medical advice, and no efficacy claim is implied. Regulatory approval is not a use recommendation by AllPeptides.eu.
⚠️ For informational/educational purposes only. Not medical advice. We do not sell any products. Self-treatment carries serious risks — consult a healthcare professional. Disclaimer
Research monograph
See the full NAD+ encyclopedia page with mechanism, evidence tier, related variants and research context.
How It Works
NAD+ is essential for sirtuins, PARPs, and CD38 — enzymes that regulate aging, DNA repair, and metabolism. Replenishing NAD+ may improve mitochondrial function.
Quick Start
- Reconstitution: Add 2.5 mL bacteriostatic water → 100 mg/mL concentration.
- Range reported in research sources: 50 mg to 100 mg once daily, increased gradually.
- Easy measurement: At 100 mg/mL, 100 mg = 100 units (1.00 mL) on a U-100 syringe.
- Storage: 2–8 °C (light-sensitive).
Ranges reported in research sources (2.5 mL = 100 mg/mL)
Route: Subcutaneous injection | Frequency: Once daily
| Week | Dose | Units (per injection) |
|---|---|---|
| Week 1 | 50 mg | 50 Units (0.50 mL) |
| Week 2 | 75 mg | 75 Units (0.75 mL) |
| Weeks 3–16 | 100 mg | 100 Units (1.00 mL) |
Research sources report subcutaneous administration once daily, increased gradually from 50 mg to 100 mg. The values are calculation examples from research sources, not validated human dosing.
Related compounds
Reconstitution Steps
- Draw 2.5 mL of bacteriostatic water.
- Inject slowly.
- Swirl gently until dissolved.
- Refrigerate at 2–8 °C, away from light.
Protocol Overview
- Goal: Enhance cellular metabolism and energy.
- Schedule reported in research sources: 50 mg to 100 mg subcutaneously, once daily, increased gradually over 16 weeks.
- Vials for the full schedule: about 43 vials of 250 mg (≈10,675 mg in total).
Dosing Protocol
- Week 1: 50 mg once daily.
- Week 2: 75 mg once daily.
- Weeks 3–16: 100 mg once daily.
- Cycle: 4–8 weeks, subcutaneous only.
- There is no validated human dosing — research context only.
- Break between cycles: After the 16-week schedule, sources describe a break of 4 weeks.
Washout & Cycle Restart
When can I restart?
After the 16-week schedule, sources describe a break of 4 weeks.
Minimum wait period: 4 weeks
Storage Instructions
- Powder: 2–8 °C, dark conditions.
- Solution: 2–8 °C, use within 14 days.
Important Notes
- Starter vial size — ideal for evaluation.
- The injection may sting (normal).
- NAD+ declines with age — exogenous administration is under investigation.
Potential Benefits & Side Effects
Potential Benefits:
- Supports cellular energy.
- Potential anti-aging effects (sirtuin activation).
- Cognitive function improvement (under investigation).
Potential Side Effects:
- Burning sensation at the injection site.
- Nausea (rare).
⚠️ Serious Warnings & Long-Term Risks
The following warnings are based on published literature, FDA labels, and post-marketing surveillance. They do not constitute medical advice. Consult a healthcare professional.
Metabolism enhancement — potential tumor protection
NAD+ elevation may theoretically benefit cancer cells by enhancing their energy metabolism (Warburg effect). Some studies show that NAMPT inhibition (NAD synthesis enzyme) suppresses oncogenesis.
📄 Cancer Cell 2018; Nat Rev Cancer 2020Flushing & GI discomfort with IV administration
IV NAD+ commonly causes chest tightness, flushing, nausea, cramps. Subcutaneous administration reduces these but with local pain.
📄 GeroScience 2023References
- Cell Metabolism — NAD+ metabolism and aging
View Source - NAD+ Restores Mitochondrial Function — Science (2013) — NAD+ restoration of mitochondrial function
View Source
Recommended Supplements
NMN (Nicotinamide Mononucleotide)
CoQ10 (Ubiquinol)
Vitamin E (Mixed Tocopherols)
External Sources & Regulatory Status
Regulatory Approvals
Dietary supplement (GRAS)
Dietary supplement (GRAS)
Educational information. Not medical advice, and no efficacy claim is implied. Regulatory approval is not a use recommendation by AllPeptides.eu.
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