TB-500 (20mg)
TB-500 is a synthetic peptide corresponding to the active region of thymosin beta-4 (Tβ4), studied for tissue repair and anti-inflammatory activity. Research-use literature describes a loading phase of 2 mg, twice weekly and maintenance of 2 mg, once weekly.
Reviewed by the AllPeptides.eu Editorial Team·Last reviewed: 2026-06-16
Educational information. Not medical advice, and no efficacy claim is implied. Regulatory approval is not a use recommendation by AllPeptides.eu.
⚠️ For informational/educational purposes only. Not medical advice. We do not sell any products. Self-treatment carries serious risks — consult a healthcare professional. Disclaimer
Research monograph
See the full TB-500 encyclopedia page with mechanism, evidence tier, related variants and research context.
How It Works
TB-500 interacts with G-actin to regulate cell migration, stem cell differentiation, and the inflammatory response. It promotes angiogenesis and healing in multiple tissue types.
Quick Start
- Reconstitution: Add 4.0 mL bacteriostatic water → 5 mg/mL concentration.
- Loading (Wks 1–4): 2 mg twice weekly (4 mg/week).
- Maintenance (Wks 5–8): 2 mg once weekly.
- Easy measurement: At 5 mg/mL, 2 mg = 40 units (0.40 mL) on a U-100 syringe.
- Storage: Lyophilized: −20 °C; reconstituted: 2–8 °C, use within 28 days.
Literature-reported ranges (4.0 mL = 5 mg/mL)
Route: Subcutaneously | Frequency: 2×/week (loading) → 1×/week
| Week | Dose | Units (per injection) |
|---|---|---|
| Weeks 1–4 (loading) | 2 mg | 40 Units (0.40 mL) |
| Weeks 5–8 (maintenance) | 2 mg | 40 Units (0.40 mL) |
Loading: twice weekly (e.g., Monday/Thursday) for 4 weeks. Maintenance: once weekly. There is no validated human dosing for TB-500.
Related compounds
Reconstitution Steps
- Draw 4.0 mL of bacteriostatic water.
- Inject slowly down the vial wall; avoid foaming.
- Swirl gently until fully dissolved — do not shake.
- Note the reconstitution date and refrigerate at 2–8 °C.
Protocol Overview
- Literature-reported scope: Tissue repair, anti-inflammatory activity and angiogenesis (mostly preclinical data).
- Literature-described schedule: Loading 2 mg × 2/week for 4 weeks, maintenance 2 mg × 1/week.
- Doses per vial: 10 × 2 mg.
Dosing Protocol
- Loading (Weeks 1–4): 2 mg × 2/week = 4 mg/week.
- Maintenance (Weeks 5–8): 2 mg × 1/week.
- Literature-described break: 4–6 weeks after the cycle.
- There is no validated human dosing — research context only.
- Break between cycles: After the 8-week cycle, the literature describes a 4–6 week break.
Washout & Cycle Restart
When can I restart?
After the 8-week cycle, the literature describes a 4–6 week break.
Minimum wait period: 4 weeks
Storage Instructions
- Lyophilized: −20 °C in dry, dark conditions.
- Reconstituted: 2–8 °C; use within 28 days.
- Avoid freeze-thaw cycles.
Important Notes
- High concentration = fewer vial changes.
- Aseptic technique for every draw.
- Human clinical data is limited.
Potential Benefits & Side Effects
Potential Benefits:
- Extended cycles without frequent vial changes.
- Anti-inflammatory and regenerative action.
- More cost-effective per mg.
Potential Side Effects:
- Injection site reactions (redness, itching).
- Transient fatigue or weakness (initial days).
- Headache (rare).
- Dizziness or flushing (rare).
- Human data is preliminary.
⚠️ Serious Warnings & Long-Term Risks
The following warnings are based on published literature, FDA labels, and post-marketing surveillance. They do not constitute medical advice. Consult a healthcare professional.
Theoretical concern: angiogenesis
Thymosin β4 regulates angiogenesis and cell migration. Theoretical concern that it may favor growth of existing tumors, though no clinical evidence exists.
📄 Ann NY Acad Sci 2007References
- Annals of the NY Academy of Sciences — Thymosin β4 and tissue healing
View Source - Expert Opin Biol Ther — TB-500 in injury models
View Source
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External Sources & Regulatory Status
Regulatory Approvals
Research-only — No regulatory approval
Educational information. Not medical advice, and no efficacy claim is implied. Regulatory approval is not a use recommendation by AllPeptides.eu.
Continue Your Research
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