GHK-Cu (100mg)
GHK-Cu dosing protocols leverage this naturally occurring copper-binding tripeptide (glycyl-L-histidyl-L-lysine) to support skin rejuvenation, wound healing, and tissue repair. GHK-Cu stimulates collagen and elastin production, promotes angiogenesis, and provides potent antioxidant and anti-inflammatory benefits. Human studies show improved skin thickness, reduced wrinkles, and enhanced dermal density. This protocol outlines a once-daily subcutaneous approach.
Reviewed by the AllPeptides.eu Editorial Team·Last reviewed: 2026-06-16
Educational information. Not medical advice, and no efficacy claim is implied. Regulatory approval is not a use recommendation by AllPeptides.eu.
⚠️ For informational/educational purposes only. Not medical advice. We do not sell any products. Self-treatment carries serious risks — consult a healthcare professional. Disclaimer
Research monograph
See the full GHK-Cu encyclopedia page with mechanism, evidence tier, related variants and research context.
How It Works
GHK-Cu acts as a 'signal peptide' that triggers tissue repair processes by chelating copper(II) ions and delivering this essential trace element to cells in a bioavailable form. Copper is a critical cofactor for enzymes in collagen synthesis (lysyl oxidase) and antioxidant defense (superoxide dismutase). Genomic studies show GHK-Cu regulates over 30% of human genes toward a regenerative, anti-aging phenotype. It stimulates VEGF and bFGF production in fibroblasts, promoting angiogenesis and blood flow to injured tissue. It also recruits immune cells, increases collagen/elastin/glycosaminoglycan synthesis, and modulates matrix metalloproteinases for balanced tissue remodeling.
Quick Start
- Reconstitution: Add 3.0 mL bacteriostatic water → ~33.3 mg/mL concentration.
- Typical Daily Range: 1–2 mg once daily (gradual titration over 12 weeks).
- Storage: Lyophilized: freeze at −20 °C; after reconstitution, refrigerate at 2–8 °C; use within 30 days.
Standard / Titration Approach (3 mL = ~33.3 mg/mL)
Route: Subcutaneous | Frequency: Once daily (5 days/week)
| Week | Dose | Units (per injection) |
|---|---|---|
| Weeks 1–4 | 1.0 mg | 3 Units (0.03 mL) |
| Weeks 5–8 | 1.5 mg | 4.5 Units (0.04 mL) |
| Weeks 9–12 | 2.0 mg | 6 Units (0.06 mL) |
Studies report administration once daily subcutaneously, 5 days/week (Mon–Fri) with weekends off.
Related compounds
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water (BAC water). Alternative: 0.6% acetic acid may improve the stability of the copper complex.
- Inject slowly down the side; avoid foaming.
- Gently swirl/rotate until dissolved (do not shake). The solution will have a slight blue tint (normal, from the copper).
- Label and refrigerate at 2–8 °C; use within 30 days.
Protocol Overview
- Objective: Support skin rejuvenation, collagen synthesis, wound healing, and anti-aging tissue repair.
- Schedule: Daily subcutaneous injections, 5 days on / 2 days off, for 8–12 weeks.
- Storage: Lyophilized frozen at −20 °C; reconstituted refrigerated; use within 30 days.
Dosing Protocol
- Initiation: 1.0 mg daily for Weeks 1–4; increase to 1.5 mg for Weeks 5–8.
- Target: 2.0 mg daily through Weeks 9–12.
- Frequency: Once daily (subcutaneously), 5 days/week.
- Cycle Duration: 8–12 weeks; take a 2–4 week break before repeating.
- Break between cycles: After the cycle (8–12 weeks), a 2–4 week break is recommended. GHK-Cu supports tissue regeneration — the pause allows for evaluation of results.
Washout & Cycle Restart
When can I restart?
After the cycle (8–12 weeks), a 2–4 week break is recommended. GHK-Cu supports tissue regeneration — the pause allows for evaluation of results.
Minimum wait period: 4 weeks
Storage Instructions
- Lyophilized: Store at −20 °C; stable for 12+ months.
- Reconstituted: Refrigerate 2–8 °C; use within 30 days; avoid freeze-thaw cycles.
- Allow vials to reach room temperature before opening.
Important Notes
- Reconstituted solution should be clear with a blue tint (from copper); discard if cloudy or discolored.
Potential Benefits & Side Effects
Potential Benefits:
- Clinical studies show increased skin thickness, improved dermal collagen density, and reduced wrinkle depth.
- Promotes angiogenesis, granulation tissue formation, and accelerated wound healing.
- Anti-inflammatory action: suppresses TNF-α, IL-6 and provides antioxidant protection.
- Enlargement of hair follicles and increased growth rate observed in studies.
- Excellent tolerability with no serious adverse events noted; extremely wide safety margin.
Potential Side Effects:
- Injection site reactions (redness, itching — transient).
- Localized erythema or skin dryness (topical use).
- Rare allergic reaction to the copper complex.
⚠️ Serious Warnings & Long-Term Risks
The following warnings are based on published literature, FDA labels, and post-marketing surveillance. They do not constitute medical advice. Consult a healthcare professional.
Angiogenesis & tissue remodeling
GHK-Cu promotes angiogenesis, tissue regeneration, and increases collagen/elastin. Theoretical concern for enhancing existing tumors, though no clinical signal exists.
📄 Life Sci 2012References
- PMC – The Potential of GHK as an Anti-Aging Peptide — GHK-Cu mechanisms: copper delivery, anti-inflammatory, tissue repair
View Source - PMC – Regenerative and Protective Actions of the GHK-Cu Peptide — Gene expression, skin remodeling, collagen synthesis
View Source - Int. J. Med. Sci. — Tripeptides in wound healing and skin regeneration
View Source - Topical GHK as Anti-Wrinkle Peptide — Clinical Evaluation — Topical GHK as an anti-wrinkle peptide
View Source - GHK-Cu in Prevention of Oxidative Stress and Degenerative Conditions (PMC) — GHK-Cu in prevention of oxidative stress and degenerative conditions
View Source
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External Sources & Regulatory Status
Regulatory Approvals
Research-only — Cosmetic (topical) use unrestricted
Educational information. Not medical advice, and no efficacy claim is implied. Regulatory approval is not a use recommendation by AllPeptides.eu.
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