BPC-157 + TB-500 + GHK-Cu (5mg + 5mg + 50mg)

The combination acts on three complementary levels: **BPC-157** activates the VEGF and NO pathways, creating new blood vessels at the injury site (angiogenesis) and accelerating the inflammatory phase. **TB-500** binds G-actin, reorganizes the cytoskeleton, and promotes cell migration (keratinocytes, fibroblasts, endothelial cells) to the site of injury. **GHK-Cu** activates genes for collagen (I, III), elastin, and proteoglycan synthesis, remodeling the extracellular matrix and enhancing the mechanical strength of the new tissue.

• BPC-157: 250–500mcg SC, 2x/day
• TB-500: 2.5mg SC, 2x/week (loading), 1x/week (maintenance)
• GHK-Cu: 1–2mg SC, 1x/day
• Cycle: 4–8 weeks

1. BPC-157 5mg: Add 2ml of bacteriostatic water → 2.5mg/ml (250mcg/0.1ml). Swirl gently.
2. TB-500 5mg: Add 2ml of bacteriostatic water → 2.5mg/ml. Swirl gently.
3. GHK-Cu 50mg: Add 2ml of bacteriostatic water → 25mg/ml (1mg/0.04ml). Swirl gently.
4. Use only bacteriostatic water (benzyl alcohol 0.9%).
5. Do not shake — swirl or let it dissolve naturally.

• This stack combines three mechanisms of tissue repair:
• BPC-157 (Body Protection Compound) — A pentadecapeptide isolated from gastric juice. Promotes angiogenesis (VEGF), enhances nitric oxide (NO), and accelerates the healing of tendons, muscles, nerves, and the GI mucosa.
• TB-500 (Thymosin Beta-4) — A 43-amino acid peptide. Regulates actin, promotes cell migration, reduces inflammation and fibrosis. Acts systemically in all tissues.
• GHK-Cu (Copper Peptide) — A tripeptide-copper complex. Enhances synthesis of collagen, elastin, and glycosaminoglycans. It is an antioxidant, anti-inflammatory, and enhances tissue remodeling.
• Synergy: BPC-157 creates new blood vessels, TB-500 transports cells to the site, and GHK-Cu rebuilds the extracellular matrix.

• Loading Phase (wks 1–4): Higher doses and frequency for rapid initiation of healing.
• BPC-157: 250–500mcg SC 2x/day. Ideally near the injury site.
• TB-500: 2.5mg SC 2x/week (e.g., Monday + Thursday).
• GHK-Cu: 1–2mg SC 1x/day, or topically in a cream/serum.
• Maintenance Phase (wks 5–8): Dose reduction as healing becomes established.
• BPC-157: 250mcg 1x/day. TB-500: 2.5mg 1x/week. GHK-Cu: 1mg 1x/day.
• Monitor pain, mobility, and swelling.
• Break between cycles: After the cycle (4–8 weeks), a pause of 4–6 weeks is recommended. Healing peptides act on growth factors (VEGF, FGF)—the pause prevents chronic overstimulation and allows for the completion of tissue remodeling.

• All peptides (powder): 2–8°C or -20°C for long-term storage.
• After reconstitution: 2–8°C (refrigerator), away from light.
• BPC-157: Use within 28 days after reconstitution.
• TB-500: Use within 28 days after reconstitution.
• GHK-Cu: Use within 21 days after reconstitution. Sensitive to light.

• Investigational stack — no clinical studies exist for this triple combination.
• BPC-157 has extensive preclinical data but limited human studies.
• TB-500 may theoretically promote the growth of existing tumors — avoid in case of cancer history.
• GHK-Cu contains copper — do not exceed recommended doses.
• Ideal for: tendon, muscle, and joint injuries; post-surgical recovery.
• Medical supervision is required, especially post-surgery.

1. BPC-157 Healing Properties — Sikiric P, et al. Stable gastric pentadecapeptide BPC 157: novel therapy in gastrointestinal tract. Curr Pharm Des. 2018;24(18):2012-2032.
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2. BPC-157 Tendon Healing — Chang CH, et al. BPC 157 enhances the growth hormone receptor expression in tendon fibroblasts. Molecules. 2014;19(11):19066-19077.
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3. TB-500 Tissue Repair — Goldstein AL, et al. Thymosin β4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol Med. 2005;11(9):421-429.
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4. TB-500 Anti-inflammatory — Sosne G, et al. Thymosin beta 4: a potential novel therapy for neurotrophic keratopathy. Ann N Y Acad Sci. 2007;1112:267-271.
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5. GHK-Cu Collagen Synthesis — Pickart L, et al. GHK peptide as a natural modulator of multiple cellular pathways in skin regeneration. Biomed Res Int. 2015;2015:648108.
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6. GHK-Cu Gene Expression — Pickart L, et al. GHK-Cu may prevent oxidative stress in skin by regulating copper and modifying expression of numerous antioxidant genes. Cosmetics. 2015;2(3):236-247.
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7. BPC-157 & Angiogenesis — Seiwerth S, et al. BPC 157 and standard angiogenic growth factors: effect on inflammation and wound healing. Curr Pharm Des. 2018;24(18):1972-1989.
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