Pinealon (20mg)

Pinealon (sequence **Glu-Asp-Arg / EDR**) is a **tripeptide bioregulator** developed by Vladimir Khavinson. It acts as an **epigenetic regulator**: it crosses the blood-brain barrier, enters the nuclei of neuronal cells, and interacts directly with DNA, regulating the **gene expression** of neuroprotective proteins. In **pinealocytes** (pineal gland cells), it enhances the synthesis of **melatonin** and **serotonin**, restoring normal circadian rhythms. In neuronal models, it reduces **oxidative stress** by increasing antioxidant enzymes (SOD, GPx) and inhibiting the formation of free radicals. Studies in elderly monkeys and humans show **restoration of nocturnal melatonin**, improvement in sleep quality, and **cognitive function**.

• Typical Dose: 1–2mg subcutaneously or intranasally
• Frequency: Daily for 10–20 days
• Storage: 2–8°C after reconstitution

1. Clean the vial stoppers with an alcohol swab.
2. Draw 2mL of bacteriostatic water.
3. Slowly inject against the side wall of the vial.
4. Gently swirl until fully dissolved.
5. Store in the refrigerator (2–8°C).

• Pinealon (EDR: Glu-Asp-Arg) is a synthetic tripeptide — a Khavinson bioregulator that targets the brain and the pineal gland.
• It was developed at the St. Petersburg Institute of Bioregulation and Gerontology by Vladimir Khavinson — a pioneer in peptide bioregulation.
• It acts as an epigenetic regulator — it crosses the blood-brain barrier and regulates gene expression in neurons.
• It enhances the production of melatonin from pinealocytes, regulating circadian rhythms and sleep.
• Low side effect profile — typical Khavinson cycle: 10–20 days, 2–3 cycles/year.

• Typical Dose: 1–2mg/day subcutaneously or intranasally.
• Cycle: 10–20 days (typical Khavinson protocol).
• Administer before sleep for optimal melatonin regulation.
• Repeat every 3–6 months (2–3 cycles/year).
• It is often combined with Cortagen (adrenal glands) or Crystagen (immune system) for holistic bioregulation.
• Break between cycles: After the cycle (10–20 days), a pause of 3–6 months is recommended. Khavinson bioregulatory peptides have a prolonged epigenetic action — gene regulation persists for months after discontinuation. Typically 2–3 cycles/year.

• Reconstituted: 2–8°C, use within 21 days.
• Lyophilized powder: 2–8°C or −20°C for long-term storage.
• Do not freeze after reconstitution.

• Neuroprotective — particularly of interest for neurodegeneration and cognitive decline.
• Low side effect profile — typical for Khavinson bioregulatory peptides.
• Regulates circadian rhythm by enhancing melatonin — ideal for administration before sleep.
• The intranasal route provides faster brain action (bypasses BBB).
• The use of this protocol is for informational purposes only.

1. Pinealon & Serotonin — Khavinson VK, et al. Short peptides stimulate serotonin release by cultured rat pinealocytes. Bull Exp Biol Med. 2012;153(1):44-46.
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2. Khavinson Peptide Bioregulation — Khavinson VK. Peptides and Ageing. Neuroendocrinol Lett. 2002;23 Suppl 3:11-144.
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3. EDR Neuroprotection — Khavinson VK, et al. Neuroprotective effect of EDR peptide in vitro. Bull Exp Biol Med. 2010;149(1):71-73.
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4. Peptide Epigenetic Regulation — Fedoreyeva LI, et al. Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry (Mosc). 2011;76(11):1210-1219.
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5. Bioregulators in Gerontology — Khavinson VK, et al. Peptide geroprotectors — epigenetic regulators. St. Petersburg Institute of Bioregulation and Gerontology, 2014.
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