Glutathione (600mg)
Glutathione dosing protocols leverage this master antioxidant tripeptide (γ-L-glutamyl-L-cysteinyl-glycine) to support cellular detoxification, reduce oxidative stress, and maintain redox balance throughout the body. As the most abundant intracellular thiol, glutathione plays a central role in neutralizing free radicals, supporting liver function, enhancing immune cell activity, and protecting against environmental toxins. This protocol outlines a subcutaneous injection approach.
Reviewed by the AllPeptides.eu Editorial Team·Last reviewed: 2026-06-16
Educational information. Not medical advice, and no efficacy claim is implied. Regulatory approval is not a use recommendation by AllPeptides.eu.
⚠️ For informational/educational purposes only. Not medical advice. We do not sell any products. Self-treatment carries serious risks — consult a healthcare professional. Disclaimer
How It Works
Glutathione (GSH) is the most abundant intracellular antioxidant, present in virtually every cell at concentrations of 1–10 mM. As a tripeptide of glutamate, cysteine, and glycine, it functions as the primary thiol-redox buffer, neutralizing reactive oxygen species (ROS), supporting Phase II detoxification pathways, and facilitating the excretion of xenobiotics and heavy metals. The reduced form (GSH) donates an electron to neutralize free radicals and is then recycled from its oxidized form (GSSG) via glutathione reductase.
Quick Start
- Reconstitution: Add 4.0 mL bacteriostatic water → 150 mg/mL concentration.
- Typical daily range: 100–200 mg subcutaneously (gradual titration).
- Storage: Lyophilized: −20 °C; post-reconstitution, refrigerate 2–8 °C; avoid freeze-thaw.
Typical / Gradual Approach (4 mL = 150 mg/mL)
Route: Subcutaneously | Frequency: Once daily
| Week | Dose | Units (per injection) |
|---|---|---|
| Weeks 1–2 | 100 mg | 66.7 Units (0.67 mL) |
| Weeks 3–4 | 150 mg | 100 Units (1.00 mL) |
| Weeks 5–8 | 200 mg | 2 × 66.7 Units (0.67 mL) |
Studies report administration once daily subcutaneously, or every other day depending on goals. Some protocols use 200 mg 1–2× weekly for maintenance.
Related compounds
Reconstitution Steps
- Draw 4.0 mL of bacteriostatic water.
- Inject slowly down the side; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label and refrigerate at 2–8 °C.
Protocol Overview
- Goal: Support antioxidant defense, cellular detoxification, and immune function.
- Schedule: Daily or every-other-day subcutaneous injections for 4–8 weeks.
- Storage: Lyophilized frozen; reconstituted refrigerated; avoid freeze-thaw.
Dosing Protocol
- Initiation: 100 mg daily; increase by ~50 mg every 1–2 weeks.
- Target: 150–200 mg daily by Wk 3–8.
- Frequency: Once daily or every other day for maintenance.
- Cycle Duration: 4–8 weeks; optional 2–4 week washout between.
- Break between cycles: After an intensive cycle (4–8 weeks), a 2–4 week washout period or transition to a maintenance dose (200 mg × 1–2/wk) is recommended.
Washout & Cycle Restart
When can I restart?
After an intensive cycle (4–8 weeks), a 2–4 week washout period or transition to a maintenance dose (200 mg × 1–2/wk) is recommended.
Minimum wait period: 2 weeks
Storage Instructions
- Lyophilized: Store at −20 °C in dry, dark conditions.
- Reconstituted: Refrigerate 2–8 °C; use within 2–3 weeks; avoid freeze-thaw.
- Glutathione is relatively unstable post-reconstitution—use promptly.
Important Notes
- 100 Unit/Injection Limit: At 150 mg/mL the 200 mg dose (1.33 mL) is split into 2 × 100 mg at separate sites.
Potential Benefits & Side Effects
Potential Benefits:
- Supports reduction in oxidative stress markers and maintains cellular redox balance.
- May enhance liver function and support hepatic detoxification pathways.
- Research suggests immunomodulatory effects, including support for natural killer (NK) cells and T-cells.
- Clinical studies in skin health show potential effects on melanin index and skin brightness.
- Generally well-tolerated; rare reports of gastrointestinal discomfort at high doses.
Potential Side Effects:
- Injection site reactions (redness, swelling).
- Abdominal discomfort or cramping.
- Headache (transient).
- Rare allergic reaction (rash, hives).
- Zinc depletion with long-term use.
⚠️ Serious Warnings & Long-Term Risks
The following warnings are based on published literature, FDA labels, and post-marketing surveillance. They do not constitute medical advice. Consult a healthcare professional.
Potential renal burden with IV use
Chronic IV glutathione at high doses may theoretically form renal oxalates. The FDA has not approved injectable glutathione.
📄 J Cosmet Dermatol 2019References
- Antioxidant Glutathione: Protective Roles and Regeneration Mechanisms — Antioxidant glutathione: protective roles and regeneration mechanisms
View Source - Glutathione and Dependent Enzymes: Coordinated Defense Against Oxidative Stress — Glutathione and dependent enzymes: coordinated defense against oxidative stress
View Source - Glutathione: Roles in Immune Function, Detoxification and Clinical Applications (PMC) — Glutathione: roles in immune function, detoxification, and clinical applications
View Source - Glutathione Efficacy in NAFLD Treatment: Open-Label Pilot Study (PMC) — Glutathione efficacy in NAFLD treatment: open-label pilot study
View Source - Glutathione Anti-Aging and Anti-Melanogenic Effects: RCT (PMC) — Glutathione anti-aging and anti-melanogenic effects: RCT
View Source
Recommended Supplements
Vitamin C (High-dose)
Zinc + Selenium
External Sources & Regulatory Status
Regulatory Approvals
Dietary supplement (GRAS)
Dietary supplement (GRAS)
Educational information. Not medical advice, and no efficacy claim is implied. Regulatory approval is not a use recommendation by AllPeptides.eu.
Continue Your Research
Explore tools and guides related to this protocol.
Important Disclaimer
The content of this website is provided exclusively for informational and educational purposes within the context of research literature.
Read the full Disclaimer →